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Isolation of peripheral blood mononuclear cells (PBMCs) and single-cell suspensions was performed using Ficoll Paque Plus (Millipore Sigma) density gradient centrifugation

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200 ml of serum was added to the sample tube, then 800 ml of phosphate buffer, 25 ml of butylated hydroxy toluene (BHT) solution and 500 ml of 30% tricarboxylic acid (TCA) were added to it

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