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The organic content was analyzed using data-dependent auto LC-MS (maXis, Bruker Daltonics) coupled to an UHPLC ACQUITY (Waters) using reverse phase chromatography (CORTECS UPLC C18 column, 150 mm 2.1 mm ID 1.6 m, Waters Corporation) in both positive and negative electrospray modes

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Mass spectrometric characterization of full-length rat selenoprotein P and three isoforms shortened at the C terminus

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