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Sterile water for injection works for single-use vials only

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Thereafter, RPMI 1640 containing 1% FBS was added to prevent proliferation

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Here is a quick storage guide Before reconstitution (powder) Storage : Refrigerate at 2-8C (36-46F) Shelf life : 2-3 years when properly stored Freezer : Can freeze for longer storage (3-5 years) Room temperature : Survives short periods (days to weeks) but refrigeration preferred After reconstitution (liquid) Storage : Refrigerate immediately at 2-8C Shelf life : 28 days with bacteriostatic water Freezer : Not recommended (can damage peptide structure) Room temperature : Begins degrading within hours, use within days max Key rules Always refrigerate reconstituted peptides Use bacteriostatic water (not sterile water) Mark reconstitution date on vial Protect from light (store in box) Use within 28 days after reconstitution Never shake vials vigorously Understanding peptide stability Why peptides degrade Chemical breakdown : Peptide bonds can break down over time, especially in liquid form