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After stirring 1 h, the solvent was removed under reduced pressure, the organic layer was extracted with ethyl acetate (15 ml), and then washed with 2 5 ml of 10% citric acid, 2 5 ml NaHCO3 (1M), and then with 2 5 ml brine

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Sericin and melatonin mitigate diethylnitrosamine-instigated testicular impairment in mice: implications of oxidative stress, spermatogenesis, steroidogenesis, and modulation of Nrf2/WT1/SF-1 signaling pathways

This condition creates an opportunity to form mtDNA-protein crosslinks mediated by ROS, which increases mitochondrial fission and exacerbates mtDNA damage (Caston and Demple, 2017

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